A key goal of the Magaliesberg Biosphere is the harmonious development of man and the conservation of nature. Biosphere reserves represent a global network of model regions in which sustainable forms of land-use and options for adaptation to changing ecological, economic and social conditions can be tested. The involvement and contribution of all communities whose activities, resources and wellbeing are already linked to the region, is central to the establishment and functioning of the biosphere.

All biosphere reserves are classified into three biosphere zones which show the gradation of human-environment interaction within them. The Magaliesberg Biosphere has two core zones, the Cradle of Humankind World Heritage Site and the Magaliesberg Protected Environment. These areas are mostly conservation areas, protected by legislation within the environmental legal framework of the country.

The biosphere buffer zone surrounds the core zones and also comprises informally protected areas such as nature reserves, conservancies and other areas that demonstrate biodiversity stewardship or have endorsed the biosphere concept. All other areas within the biosphere make up the transition zone, mostly the built up areas with a diversity of economic activity.

In all three zones, various strategies and guidelines are developed to manage the complexity of people, development and nature sustainably.